DNA Extraction and Purification
Biotium Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (CALD1/1424R), CF647 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low
Zymo Research Corporation Quick-DNA™ Urine DNA Kit (50 Preps) w/ Zymo-Spin™ IC-S Columns (Capped)
The product features a uniquely formulated urine DNA stabilization reagent that also functions as a precipitation reagent. After collection, total (cellular and cell-free) or cell-free urine can be stored at ambient temperature for up to one month by adding the Urine Conditioning Buffer. When ready to extract the urine DNA, just add the Clearing Beads, vortex, and centrifuge to collect the precipitate. Following precipitation, chemical lysis and enzymatic digestion are used to extract DNA from the precipitate. The DNA is subsequently purified and concentrated using ZymoSpin™ IC-S Columns. Urine DNA isolated with the Quick-DNA™ Urine Kit is ideal for qPCR, array, methylation analysis, and other downstream applications.
![Zymo Research Corporation Zymoprep™ -96 Yeast Plasmid Miniprep (2 x 96 preps) [Includes E1004 x 2: Zymolyase (1 x 2000 Units Lyophilized) w/ Storage Buffer (500µl)]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D2005ZYMOPREPYEASTPLASMIDMINI.JPG-250.jpg)
Zymo Research Corporation Zymoprep™ -96 Yeast Plasmid Miniprep (2 x 96 preps) [Includes E1004 x 2: Zymolyase (1 x 2000 Units Lyophilized) w/ Storage Buffer (500µl)]
The Zymoprep™-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep™-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase™ added in the first solution. There is no need for glass beads, or phenol.
Zymo Research Corporation ZymoPURE Wash 1, 410 ml
ZymoPURE Wash 1 is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Biochain Institute Inc Control Genomic DNA - Chicken Male, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
New England Biolabs, Inc. NEBNext Ultra™ II FS DNA Module – 96 reactions
This module is part of the NEBNext Ultra II FS workflow, and is optimized for use with the NEBNext Ultra II Ligation Module, for Illumina-compatible library construction.
Biochain Institute Inc Control Genomic DNA - Human Male, 100 ug/PK
BioChain's control genomic DNAs are isolated from tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >;2.0. Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Beckman Coulter DNA Isolation From FFPE Tissue
The Genomic DNA Isolation Kit from Beckman Coulter is designed to isolate genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. It uses a column-based method to purify DNA, removing cross-links, inhibitors, and contaminants. The kit includes a microtube pestle for lysing samples, Proteinase K for digestion, Lysis Buffer, Wash Buffers, Elution Buffer, and Spin Columns. It can process up to 4 x 25um sections per prep, yields up to 12 μg DNA per sample, and produces DNA ready for PCR, Southern blotting, and other applications. The streamlined procedure takes around 90 minutes.
Electron Microscopy Sciences NSA (Nonenyl Succinic Anhydride) 450 ML
F.W. 227
Boiling point: 325 °C (617 °F)
An epoxy hardener that is specially distilled. The special distillation process offers you much clearer blocks than the standard NSA
LGC GENOMICS LLC (Lucigen) MasterPure Gram Positive DNA Purification Kit, 100 PurifPurify genomic DNA from challenging Gram-positive bacteria for a wide variety of molecular biology applications.
The MasterPure™ Gram-Positive DNA Purification Kit provides all of the reagents needed to purify DNA from Gram-positive bacteria.
Agilent Technologies DNA ScreenTape Analysis, 1 each
D1000 Reagents. For the analysis of DNA from 35 to 1000 bp. Includes ladder and sample buffer sufficient for 112 samples.
Bulldog Bio Inc FastGene Scriptase II ReadyMix (100 rxns), Random Hex-primers
How would you construct the ideal reverse transcriptase? It would have to make very long cDNAs at high yields. It would have to be able to work from very small amounts of input RNA. And of course, it would have to work fast. FastGene Scriptase II checks all three boxes, and it does it at a reasonable price. Here’s how. First, mutations are inserted into the RNase H domain of native MuLV‘s reverse transcriptase delivering a higher yield of full-length cDNAs. Next, changes are made to increase the enzymes natural thermal stability providing a more robust enzyme that doesn’t lose processing power, even at elevated temperatures. Finally, the reaction buffer composition is optimized for high speed production of cDNAs from incredibly low levels of input RNA. This unique combination of size, speed, and production makes FastGene Scriptase II the optimal choice for even the most complex applications requiring cDNAs, such as RT-qPCR and NGS.
Takara Bio NucleoBond® Xtra Midi EF, 50 preps
NucleoBond Xtra Midi EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A.